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NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish

Mattern, Kai GND; Trotha, Jakob William von ORCID; Erfle, Peer ORCID; Köster, Reinhard Wolfgang ORCID; Dietzel, Andreas ORCID

While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease.

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