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The First Step of Neurospora crassa Molybdenum Cofactor Biosynthesis : Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions

GND
1209172208
Affiliation
TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, Germany.
Wajmann, Simon;
GND
1209173018
Affiliation
TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, Germany.
Hercher, Thomas W.;
GND
1135189617
Affiliation
TU Braunschweig, Institute of Physical and Theoretical Chemistry, Antibody Facility, 38106 Braunschweig, Germany.
Buchmeier, Sabine;
GND
115476784
Affiliation
TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, Germany.
Hänsch, Robert;
GND
10945698X
Affiliation
TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, Germany.
Mendel, Ralf R.;
GND
139372873
Affiliation
TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, Germany.
Kruse, Tobias

Molybdenum cofactor (Moco) is the active site prosthetic group found in all Moco dependent enzymes, except for nitrogenase. Mo-enzymes are crucial for viability throughout all kingdoms of life as they catalyze a diverse set of two electron transfer reactions. The highly conserved Moco biosynthesis pathway consists of four different steps in which guanosine triphosphate is converted into cyclic pyranopterin monophosphate, molybdopterin (MPT), and subsequently adenylated MPT and Moco. Although the enzymes and mechanisms involved in these steps are well characterized, the regulation of eukaryotic Moco biosynthesis is not. Within this work, we described the regulation of Moco biosynthesis in the filamentous fungus Neurospora crassa, which revealed the first step of the multi-step pathway to be under transcriptional control. We found, that upon the induction of high cellular Moco demand a single transcript variant of the nit-7 gene is increasingly formed pointing towards, that essentially the encoded enzyme NIT7-A is the key player for Moco biosynthesis activity in Neurospora.

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